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Our newly-generated sequences were aligned by locus with ex-type and other representative sequences of Zasmidium species, which were downloaded from GenBank (Table 1). Alignments were made using the online version of MAFFT v. 7.307 (Katoh and Standley 2016) and manually improved, where necessary, using MEGA v. 6.06 (Tamura et al. 2013). Mesquite v. 2.75 (Maddison 2008) was used to concatenate the aligned sequences of the different loci. Ambiguous regions were excluded from analyses using AliView (Larsson 2014) and gaps were treated as missing data. DNA base differences of different gene loci between our strains and ex-type or representative strains of relative taxa are shown in Table 2. The alignment document is available in TreeBASE under the study ID 27250.

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